Principles of light microscopy pdf

Light travels through the sample under a conventional microscope as far into the specimen as it can penetrate, while a confocal microscope only focuses a smaller beam of light at one narrow depth level at a time. 3D imaging and surface profiling of samples. The fluorescence is visible by confocal microscopy. However, the actual dye concentration can be low to minimize the disturbance of biological systems: some instruments principles of light microscopy pdf track single fluorescent molecules.